Howlett, I. D., Han, W., Rice, P., Barton, J. K., & Kostuk, R. K. (2017). Wavelength-coded volume holographic imaging endoscope for multidepth imaging. Journal of biomedical optics, 22(10), 1-4.
A wavelength-coded volume holographic imaging (WC-VHI) endoscope system capable of simultaneous multifocal imaging is presented. The system images light from two depths separated by 100 μm in a tissue sample by using axial chromatic dispersion of a gradient index probe in combination with two light-emitting diode sources and a multiplexed volume hologram to separate the images. This system is different from previous VHI systems in that it uses planar multiplexed gratings and does not require curved holographic gratings. This results in improved lateral imaging resolution from 228.1 to 322.5 lp/mm. This letter describes the design and fabrication of the WC-VHI endoscope and experimental images of hard and soft resolution targets and biological tissue samples to illustrate the performance properties.
Tate, T., Keenan, M., Swan, E., Black, J., Utzinger, U., & Barton, J. (2014). Optical design of an optical coherence tomography and multispectral fluorescence imaging endoscope to detect early stage ovarian cancer. INTERNATIONAL OPTICAL DESIGN CONFERENCE 2014, 9293.
Marion, S. L., Watson, J., Sen, N., Brewer, M. A., Barton, J. K., & Hoyer, P. B. (2013). 7,12-dimethylbenz[a]anthracene-induced malignancies in a mouse model of menopause. Comparative medicine, 63(1), 6-12.
Ovarian cancer has a high mortality rate because there are few symptoms in early disease development. The incidence of ovarian cancer increases in women after menopause. Understanding early events in this disease can best be achieved by using animal models. Therefore, the objective of this study was to develop and track the onset of ovarian tumorigenesis in mice mimicking characteristics of postmenopausal epithelial cancer in women. Female B6C3F1 mice (age, 28 d) received 4-vinylcyclohexene diepoxide (VCD, 160 mg/kg IV daily for 20 d) to cause ovarian failure. Four months after VCD treatment, via surgical intervention, each mouse received a single injection of 7,12-dimethylbenz[a]anthracene (DMBA) or vehicle control (sesame oil) under the bursa of the right ovary to cause ovarian neoplasms. The experimental groups were untreated controls (Con-Con), DMBA-treatment only (Con-DMBA), VCD treatment only (VCD-Con), and VCD+DMBA-treated (VCD+DMBA) mice. At 3, 5, 7, and 9 mo after DMBA injection, ovaries were collected for histologic and immunohistochemical evaluation. No tumors developed in Con-Con mice. All VCD-treated mice (with or without DMBA) exhibited ovarian failure. Mice that received both VCD and DMBA exhibited tumors at 3 mo (50%), 5 mo (14%), 7 mo (90%), and 9 mo (57%) after DMBA treatment; 31% of the tumors were epithelial in origin. Our findings confirm that inducing ovarian tumors in mice by chemical means is an effective method for studying early stages of tumor development that may be relevant to epithelial ovarian cancers that arise in postmenopausal women.
Bonnema, G. T., Cardinal, K. O., Williams, S. K., & Barton, J. K. (2009). A concentric three element radial scanning optical coherence tomography endoscope. JOURNAL OF BIOPHOTONICS, 2(6-7), 353-356.
Vargas, G., Barton, J. K., & Welch, A. J. (2008). Use of hyperosmotic chemical agent to improve the laser treatment of cutaneous vascular lesions. JOURNAL OF BIOMEDICAL OPTICS, 13(2).