David W Galbraith

Professor, Plant Science

Professor, Biomedical Engineering

Professor, BIO5 Institute

Primary Department

School of Plant Sciences

Department Affiliations

School of Plant Sciences

BIO5 Institute

Contact

(520) 621-9153

galbraith@arizona.edu

Work Summary

I examine the molecular functions of the different cells found in the tissues and organs of plants and animals and how they combine these functions to optimize the health and vigor of the organism.

Research Interest

David Galbraith obtained undergraduate and graduate degrees in Biochemistry from the University of Cambridge, and postdoctoral training as a NATO Fellow at Stanford University. His first academic appointment was at the University of Nebraska Lincoln, and he became Professor of Plant Sciences at the University of Arizona in 1989. His research has focused on the development of instrumentation and methods for the analysis of biological cells, organs, and systems. He is internationally recognized as a pioneer in the development and use of flow cytometry and sorting in plants, developing widely-used methods for the analysis of genome size and cell cycle status, and for the production of somatic hybrids. He also was among the first to develop methods for the analysis of gene expression within specific cell types, using markers based on Fluorescent Protein expression for flow sorting these cells, and microarray platforms for analysis of their transcriptional activities and protein complements. Current interests include applications of highly parallel platforms for transcript and protein profiling of minimal sample sizes, and for analysis of genetic and epigenetic mechanisms that regulate gene expression during normal development and in diseased states, specifically pancreatic cancer. He is also funded to study factors involved in the regulation of bud dormancy in Vitis vinifera, and has interests in biodiversity and improvement of third-world agriculture. He has published more than 180 scholarly research articles, holds several patents, was elected a Fellow of the American Association for Advancement of Science in 2002, and serves on the editorial board of Cytometry Part A. He is widely sought as a speaker, having presented over 360 seminars in academic, industrial and conference settings. He was elected Secretary of the International Society for Advancement of Cytometry in 2016. Keywords: Plant and Animal Cellular Engineering; Biological Instrumentation; Flow Cytometry and Sorting

Publications

The genome of flax (Linum usitatissimum) assembled de novo from short shotgun sequence reads

Wang, Z., Hobson, N., Galindo, L., Zhu, S., Shi, D., McDill, J., Yang, L., Hawkins, S., Neutelings, G., Datla, R., Lambert, G., Galbraith, D. W., Grassa, C. J., Geraldes, A., Cronk, Q. C., Cullis, C., Dash, P. K., Kumar, P. A., Cloutier, S., , Sharpe, A. G., et al. (2012). The genome of flax (Linum usitatissimum) assembled de novo from short shotgun sequence reads. PLANT JOURNAL, 72(3), 461-473.

ABA catabolism enhances dormancy release of grapevine buds.

Zheng, C., Acheampong, A., Shi, Z., Mugzech, A., Halaly, T., Shaya, F., Colova, V., Ophir, R., Galbraith, D. W., & Or, E. (2017). ABA catabolism enhances dormancy release of grapevine buds.. Plant Cell & Environment.

Flow sorting and culture of protoplasts: Conditions for high-frequency recovery, growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana

Galbraith, D. W., Afonso, C. L., & Harkins, K. R. (1984). Flow sorting and culture of protoplasts: Conditions for high-frequency recovery, growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana. Plant Cell Reports, 3(4), 151-155.

PMID: 24253474;Abstract:

Protoplasts were prepared from suspension cultures of Nicotiana tabacum cv Wisconsin 38 that had been prelabeled with FITC. The protoplasts were subjected to flow sorting based on fluorescence content using a Coulter EPICS V Flow Cytometer - Cell Sorter. Conditions were established that allowed the recovery after sorting of approximately 30% of the initial protoplasts in a viable state. These were subsequently regenerated into calli that underwent shoot morphogenesis. © 1984 Springer-Verlag.

A probable Na+(K+)/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana

Song, C. P., Guo, Y., Qiu, Q. S., Lambert, G., Galbraith, D. W., Jagendorf, A., & Zhu, J. K. (2004). A probable Na+(K+)/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101(27), 10211-10216.

Nuclear Cytometry: Analysis of the patterns of DNA synthesis and transcription using flow cytometry, confocal microscopy, and RNA sequencing.

Galbraith, D. W., Samadder, P., & Sliwinska, E. (2018). Nuclear Cytometry: Analysis of the patterns of DNA synthesis and transcription using flow cytometry, confocal microscopy, and RNA sequencing.. Methods in Molecular Biology, 1678, 371-392. doi:doi: 10.1007/978-1-4939-7346-0_16.

David W Galbraith