Pan, H., Chu, D., Yan, W., Su, Q., Liu, B., Wang, S., Wu, Q., Xie, W., Jiao, X., Li, R., Yang, N., Yang, X., Xu, B., Brown, J., Zhou, X., & Zhang, Y. (2012). Rapid spread of Tomato yellow leaf curl virus in China is aided differentially by two invasive whitefly biotypes. PLoS ONE, 7(4).
e34817. doi:10.1371/.
Chu, D., Tao, Y., Zhang, Y., Wan, F., & Brown, J. K. (2012). Effects of host, temperature and relative humidity on competitive displacement of two invasive Bemisia tabaci biotypes [Q and B]. Insect Science, 19(5), 595-603.
Abstract:
Bemisia tabaci shifted unexpectedly in China from a predominance of B biotype to Q biotype during 2005-2008. This observation stimulated an interest in investigating whether environmental factors, including host, temperature and relative humidity (RH) could possibly explain the observed shift in biotypes distribution. Results indicated that all three parameters examined influenced biotype survivability. The percentage of B biotype, when reared together on pepper plants with the Q biotype, decreased significantly from 66.7% in the founder population, to 13.6% and 3.7% in the first and second generations, respectively. When the B (founder at 66.7%) and Q (founder at 33.3%) biotypes were reared together on eggplant alone, or on pepper-plus-eggplant combination, the population size of the B biotype either remained constant, or increased somewhat in the first and second generations. On eggplant, the effects of RH and temperature on the competitiveness between the Q and B biotypes (3 pairs of Q and 6 pairs of B) were not significant. © 2012 Institute of Zoology, Chinese Academy of Sciences.
Morin, S., Henderson, S., Fabrick, J. A., Carrière, Y., Dennehy, T. J., Brown, J. K., & Tabashnik, B. E. (2004). DNA-based detection of Bt resistance alleles in pink bollworm. Insect Biochemistry and Molecular Biology, 34(11), 1225-1233.
PMID: 15522618;Abstract:
Evolution of resistance by pests is the main threat to long-term insect control by transgenic crops that produce Bacillus thuringiensis (Bt) toxins. We previously identified three mutant alleles (r1, r2, r3) of a cadherin gene in pink bollworm (Pectinophora gossypiella) linked with recessive resistance to Bt toxin Cry1Ac and survival on transgenic Bt cotton. Here we describe a polymerase chain reaction (PCR)-based method that detects the mutation in genomic DNA of each of the three resistant alleles. Using primers that distinguish between resistant and susceptible (s) alleles, this method enables identification of 10 genotypes (r1r1, r1r2, r1r3, r2r2, r2r3, r3r3, r1s, r2s, r3s, and ss) at the cadherin locus. For each of the three resistant alleles, the method detected the resistance allele in a single heterozygote (r1s, r2s, or r3s) pooled with DNA from the equivalent of 19 susceptible (ss) individuals. The results suggest that the DNA-based detection method described here could greatly increase the efficiency of monitoring for resistance to Cry1Ac compared to bioassays that detect rare individuals with homozygous resistance. © 2004 Elsevier Ltd. All rights reserved.
Brown, J. K. (2017). Occurrence of Pepper mild mottle virus (PMMoV) in groundwater from a karst aquifer system in the Yucatan Peninsula, Mexico.. Food and Environmental Virology, 9(4), 487-497. doi:10.1007/s12560-017-9309-1.
Brown, J. K. (2014). First record of Jack Beardsley mealybug, Pseudococcus jackbeardsleyi (Hemiptera: Pseudococcidae), from Africa.. Flor. Entomol. Soc., 97, 1690-1693.
N’Guessan, P.W., Watson, G.W., Brown, J.K., N'Guessan, F.K. 2014. First record of Jack Beardsley mealybug, Pseudococcus jackbeardsleyi (Hemiptera: Pseudococcidae), from Africa. Flor. Entomol. Soc. 97:1690-1693.
