Physical sciences

Walter T. Klimecki, DVM, PhD, is an Associate Professor in the Department of Pharmacology and Toxicology in the College of Pharmacy at the University of Arizona. Dr. Klimecki holds joint appointments in the College of Medicine, the College of Public Health, and the Arizona Respiratory Center. He is a Full Member of the Southwest Environmental Health Sciences Center (SWEHSC) where, together with BIO5 director Martinez and BIO5 Statistics Consulting Service director Billheimer, he leads the Integrative Health Sciences (IHS) Center at SWEHSC. The IHS is a translational research support core at SWEHSC, focused on lowering the “activation energy” for translational research.Dr. Klimecki’s research focuses on the toxicology of metals in the environment, an issue particularly relevant in our mining-intensive state. His research work has encompassed a wide range of experimental approaches, from epidemiological studies of arsenic-exposed human populations, to laboratory models including cell culture and rodents. Using cutting edge genetics tools, Dr. Klimecki’s group recently published the first report of an association between human ancestry and response to environmental toxicants. In this provocative work, his group found that individuals whose genomes were comprised of DNA with its origins in the indigenous American populations processed ingested arsenic in a less harmful manner than did individuals whose genomes had their origins in Europe. Using laboratory models his group made ground-breaking discoveries of the impact of arsenic exposure on a process known as autophagy, in which cells digest parts of their own machinery in a sort of “cash for clunkers” arrangement. The ability of arsenic to perturb this process is only now being appreciated by the toxicology community, thanks to the work of the Klimecki Lab. Dr. Klimecki was recently elected as a Vice President-elect to the Metals Specialty Section of the Society of Toxicology, the preeminent scientific toxicology organization in the world. Dr. Klimecki’s research is highly collaborative: his grants and publications have included many BIO5 members, including BIO5 director Fernando Martinez, and BIO5 members Donata Vercelli, Dean Billheimer, and Marilyn Halonen.

My research is focused on developing novel optical microscopy technologies and improving patient care using these technologies. My research area includes (1) low-cost smartphone in vivo microscopy, (2) high-speed comprehensive in vivo endomicroscopy, and (3) ultraminiature endomicroscopy. (1) Low-cost smartphone in vivo microscopy: I am currently leading a NIH-sponsored research project for developing smartphone confocal microscope and diagnosing Kaposi's sarcoma in Uganda with the smartphone confocal microscope. I will further advance the smartphone microscopy technology and address other applications, including diagnosis of cervical and oral cancers in low-resource settings, large-population screening of skin cancers in the US, and aiding science and medical educations. (2) High-speed comprehensive in vivo endomicroscopy: I have previously developed a high-speed confocal microscopy system and endoscopic imaging catheters and acquired largest in vivo confocal images of human organ reported. At the UA, I plan to further advance the technology by i) increasing the imaging speed by orders of magnitude and ii) incorporating fluorescence imaging modality. (3) Ultraminiature endomicroscopy: In my previous research, I have developed miniature endoscopic catheters that can visualize internal organs in vivo through a needle-sized device. At the UA, I will develop microscopic imaging catheter with a extremely small diameter and utilize it for guiding cancer diagnosis and treatment.

Ultrafast Electron Microscopy is a pivotal tool for imaging the atomic motion in real time and space. The temporal resolution, limited to a few hundreds of femtoseconds (one quadrillionth of a second) permits recording movies of only the relatively massive atomic motion. Imaging of microscopic motions outside the atomic nucleus in the real-time requires a significant enhancement in the temporal resolution. My research program aims to obtain the attosecond (one quintillionth of a second) temporal resolution in electron microscopy and establish the “Attomicroscopy” —the fastest camera ever known—which takes the field of ultrafast imaging to the next level. Attomicroscopy provides a real-time access to all microscopic motions outside the atomic core and radically change our insight into the workings of the microcosm. We will use the Attomicroscopy to image the electron motion in biochemical molecules such as amino acids, DNA, protein…. etc. Attosecond imaging and controlling of the electron motion at the atomic scale will open exciting new ground and prospects in multiple fields of basic science, biological applications, and information technology.

Jefferey L. Burgess, MD, MS, MPH is a Professor and Division Director of Community, Environment and Policy within the University of Arizona Mel and Enid Zuckerman College of Public Health. Dr. Burgess’ research focuses on improving occupational health and safety, with a special focus on firefighters, other public safety personnel and miners. Areas of current and past research include: reduction of occupational exposures, illnesses and injuries; respiratory toxicology; environmental arsenic exposure; and hazardous materials exposures including methamphetamine laboratories. In addition to multiple research grants, Dr. Burgess is the Principal Investigator (PI) for the Centers for Disease Control and Prevention-funded Mountain West Preparedness and Emergency Response Learning Center and a joint PI for the National Institute for Occupational Safety and Health-funded Western Mining Safety and Health Resource Center. Dr. Burgess is internationally recognized for his research evaluating the health effects of firefighting and methods for reducing firefighter exposures and other hazards, including but not limited to improved respiratory protection and injury prevention. He is also internationally known for his work on mining health and safety, and is a co-PI on a large Science Foundation Arizona grant supporting mining risk management, exposure assessment and control and economic analysis of health and safety systems. A separate ongoing grant is focused on comparing exposures and health effects associated with the use of diesel and biodiesel blend fuels in underground mining. He also has carried out multiple research projects on the adverse effects of low-level arsenic exposure in drinking water and more recently has begun to evaluate exposures from dietary arsenic sources.

Michael F. Brown is Professor of Chemistry & Biochemistry at the University of Arizona. He is co-director of the Biological Physics Program and the Chemical Physics Program, and was a co-founder of the Biological Chemistry Program at the University of Arizona. He is internationally renowned for his work on the molecular basis of activation of G-protein-coupled receptors that are the targets for the majority of pharmaceuticals and medicines used by humans. The focus of his work is on biomembranes, with a particular emphasis on lipid-protein interactions in relation to potential drug targets involving membrane proteins. He is involved with investigation of the molecular basis of visual signaling involving rhodopsin. Moreover, Professor Brown is an expert in nuclear magnetic resonance (NMR) spectroscopy. His activities in the area of biomolecular NMR spectroscopy involve the devolvement and application of methods for studying the structure and dynamics of biomolecules. Michael Brown has authored over 130 original research papers, 10 book chapters, 4 book reviews, and has published more than 275 abstracts. His current H-index is 43. He numbers among his coworkers various prominent scientists worldwide. He presents his work frequently at national and international conferences, and is the recipient of a number of major awards. Professor Brown's many contributions have established him as a major voice in the area of biomembrane research and biomolecular spectroscopy. He is frequently a member of various review panels and exerts an influence on science policy at the national level. Among his accolades, he is an elected Fellow of the American Association for the Advancement of Science; American Physical Society; Japan Society for the Promotion of Science; and the Biophysical Society. He is a Fellow of the Galileo Circle of the University of Arizona. Most recently, he received the Avanti Award of the Biophysical Society. This premier honor recognizes his vast and innovative contributions to the field of membrane biophysics, and groundbreaking work in the development of NMR techniques to characterize lipid structure and dynamics. Most recently he presented the 2014 Avanti lecture of the Biophysical Society.