Agrawal, A., Huang, S., Lin, A., Lee, M., Barton, J. K., Drezek, R. A., & Pfefer, T. J. (2006). Quantitative evaluation of optical coherence tomography signal enhancement with gold nanoshells. JOURNAL OF BIOMEDICAL OPTICS, 11(4).
Castro, J. M., Gelsinger-Austin, P. J., Barton, J. K., & Kostuk, R. K. (2011). Confocal-rainbow volume holographic imaging system. Applied optics, 50(10), 1382-8.
The performance of broadband volume holographic imaging system in terms of depth selectivity is investigated. The mechanism for depth resolution degradation is explained. In order to overcome this resolution degradation, a novel imaging device, the confocal-rainbow volume holographic imaging system, is proposed. Modeling and experimental validation of the performance of this novel imaging system indicates that depth resolution 16 μm is achievable. The lateral resolution of this device is 2.5 μm along a field of view of 300 μm×100 μm.
Barton, J., McNally, J. B., Kirkpatrick, N. D., Hariri, L. P., Tumlinson, A. R., Besselsen, D. G., Gerner, E. W., Utzinger, U., & Barton, J. K. (2006). Task-based imaging of colon cancer in the Apc(Min/+) mouse model. Applied optics, 45(13).
Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.
Barton, J., Bonnema, G. T., Cardinal, K. O., McNally, J. B., Williams, S. K., & Barton, J. K. (0). Assessment of blood vessel mimics with optical coherence tomography. Journal of biomedical optics, 12(2).
Optical coherence tomography (OCT) is an imaging modality that enables assessment of tissue structural characteristics. Studies have indicated that OCT is a useful method to assess both blood vessel morphology and the response of a vessel to a deployed stent. We evaluated the ability of OCT to visualize the cellular lining of a tissue-engineered blood vessel mimic (BVM) and the response of this lining to a bare metal stent. We develop a side-firing endoscope that obtains intraluminal, longitudinal scans within the sterile bioreactor environment, enabling time-serial assessment. Seventeen BVMs are imaged with the endoscopic OCT system. The BVMs are then evaluated via fluorescence microscopy and/or standard histologic techniques. We determine that (1) the OCT endoscope can be repeatedly inserted without visible damage to the BVM cellular lining, (2) OCT provides a precise measure of cellular lining thickness with good correlation to measurements obtained from histological sections, and (3) OCT is capable of monitoring the accumulation of cellular material in response to a metallic stent. Our studies indicate that OCT is a useful technique for monitoring the BVM cellular lining, and that OCT may facilitate the use of BVMs for early stage device assessment.
Swan, E., Tate, T., Keenan, M., Black, J. F., Utzinger, U., & Barton, J. (2015). Stray light mitigation in a novel endoscope for fallopian tubes. ENDOSCOPIC MICROSCOPY X; AND OPTICAL TECHNIQUES IN PULMONARY MEDICINE II, 9304.
