Raina Margaret Maier
Publications
Abstract:
Column studies were used to investigate the fate of a representative nonaqueous-phase liquid (NAPL), hexadecane, with specific regard to (1) the effect of attached bacteria on the formation of residual saturation and (2) the role of biodegradation and biosurfactants on the removal of residual NAPL. Residual saturation of hexadecane was determined using sterile sand (40/50 mesh) columns and was found to be 19.0 ± 4.8% of the pore volume. Columns loaded with bacterial biomass (Pseudomonas aeruginosa ATCC 15442, 109 cells g-1) showed no difference in residual hexadecane formation as compared to sterile sand columns. In further column studies examining the effect of ATCC 15442 and biosurfactants on the removal of hexadecane residual, results showed that biodegradation alone removed approximately 50% of the [14C]hexadecane, in the form of 14CO2 and undefined cellular metabolites, during elution with at least 200 pore volumes of mineral salts medium. The columns were then eluted with 1 mM rhamnolipid biosurfactant, which increased total removal to 65%. Rhamnolipid addition resulted in (1) the mobilization of hexadecane free product and (2) a transitory 3-12-fold increase in the rate of hexadecane mineralization. In a separate study, the column was eluted from the beginning with a low (0.1 mM) concentration of rhamnolipid. This lower concentration of biosurfactant enhanced the removal of hexadecane by mobilization, but had no effect on the rate of biodegradation of residual hexadecane. Analysis of residual radioactivity within two columns revealed only 2% remaining as intact hexadecane. These results suggest that a combination of biodegradation and rhamnolipid treatment could be used to maximize the removal of residual NAPL from porous media.
Past mining activities in northern Mexico left a legacy of delerict landscapes devoid of vegetation and seasonal formation of salt efflorescence. Metal content was measured in mine tailings, efflorescent salts, soils, road dust, and residential soils to investigate contamination. Climatic effects such as heavy wind and rainfall events can have great impact on the dispersion of metals in semi-arid areas, since soils are typically sparsely vegetated. Geochemical analysis of this site revealed that even though total metal content in mine tailings was relatively low (e.g. Cu= 1000 mg kg(-1)), metals including Mn, Ba, Zn, and Cu were all found at significantly higher levels in efflorescence salts formed by evaporation on the tailings impoundment surface following the rainy season (e.g. Cu= 68,000 mg kg(-1)). Such efflorescent fine-grained salts are susceptible to wind erosion resulting in increased metal spread to nearby residential soils. Our results highlight the importance of seasonally dependent salt-formation and wind erosion in determining risk levels associated with potential inhalation or ingestion of airborne particulates originating from contaminated sites such as tailings impoundments. In low metal-content mine tailings located in arid and semi-arid environments, efflorescence salts could represent a human health risk and a challenge for plant establishment in mine tailings.
PMID: 12915147;Abstract:
Heavy metal-mediated toxicity in the environment is dependent on bioavailable metal concentrations both internal and external to microbial cells. Both internal and external metal bioavailability are influenced by multiple factors in the soil environment. External factors include pH, redox potential, ionic strength, organic matter and clay content. The internal bioavailable metal concentration is dependent on both the aforementioned external factors, as well as metal uptake and efflux activities that are specific for each microorganism. The metal-specific biosensors discussed in this article can be used to measure internal metal bioavailability. © 2003 Elsevier Inc. All rights reserved.
PMID: 9293014;PMCID: PMC168669;Abstract:
The objective of this research was to evaluate the effect of low concentrations of a rhamnolipid biosurfactant on the in situ biodegradation of hydrocarbon entrapped in a porous matrix. Experiments were performed with sand-packed columns under saturated flow conditions with hexadecane as a model hydrocarbon. Application of biosurfactant concentrations greater than the CMC (the concentration at which the surfactant molecules spontaneously farm micelles or vesicles [0.03 mM]) resulted primarily in the mobilization of hexadecane entrapped within the sand matrix. In contrast, application of biosurfactant concentrations less than the CMC enhanced the in situ mineralization of entrapped hexadecane; however, this effect was dependent on the choice of bacterial isolate. The two Pseudomonas isolates tested, R4 and ATCC 15524, were used because they exhibit different patterns of biodegradation of hexadecane, and they also differed in their physical response to rhamnolipid addition. ATCC 15524 cells formed extensive multicell aggregates in the presence of rhamnolipid while R4 cells were unaffected. This behavior did not affect the ability of the biosurfactant to enhance the biodegradation of hexadecane in well-mixed soil slurry systems but had a large affect on the extent of entrapped hexadecane biodegradation in the sand-packed-column system that was used in this study.