Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., Galbraith, D., & Bohnert, H. J. (2001). Gene expression profiles during the initial phase of salt stress in rice. Plant Cell, 13(4), 889-905.
PMID: 11283343;PMCID: PMC135538;Abstract:
Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defense-related functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.
Blaszczak, A. G., Galbraith, D. W., Janda, J., Vanier, C., Smith, R., Gutierrez, A., & Wozniak, E. (2016). Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress.. Acta Horticulturae, 1148, 85-92. doi:doi: 10.17660/ActaHortic.2016.1148.10
Inan, G., Zhang, Q., Li, P. H., Wang, Z. L., Cao, Z. Y., Zhang, H., Zhang, C. Q., Quist, T. M., Goodwin, S. M., Zhu, J. H., Shi, H. H., Damsz, B., Charbaji, T., Gong, Q. Q., Ma, S. S., Fredricksen, M., Galbraith, D. W., Jenks, M. A., Rhodes, D., , Hasegawa, P. M., et al. (2004). Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. PLANT PHYSIOLOGY, 135(3), 1718-1737.
Bohnert, H. J., Ayoubi, P., Borchert, C., Bressan, R. A., Burnap, R. L., Cushman, J. C., Cushman, M. A., Deyholos, M., Fischer, R., Galbraith, D. W., Hasegawa, P. M., Jenks, M., Kawasaki, S., Koiwa, H., Kore-eda, S., Lee, B. -., Michalowski, C. B., Misawa, E., Nomura, M., , Ozturk, N., et al. (2001). A genomics approach towards salt stress tolerance. Plant Physiology and Biochemistry, 39(3-4), 295-311.
Abstract:
Abiotic stresses reduce plant productivity. We focus on gene expression analysis following exposure of plants to high salinity, using salt-shock experiments to mimic stresses that affect hydration and ion homeostasis. The approach includes parallel molecular and genetic experimentation. Comparative analysis is employed to identify functional isoforms and genetic orthologs of stress-regulated genes common to cyanobacteria, fungi, algae and higher plants. We analyze global gene expression profiles monitored under salt stress conditions through abundance profiles in several species: in the cyanobacterium Synechocystis PCC6803, in unicellular (Saccharomyces cerevisiae) and multicellular (Aspergillus nidulans) fungi, the eukaryotic alga Dunaliella salina, the halophytic land plant Mesembryanthemum crystallinum, the glycophytic Oryza sativa and the genetic model Arabidopsis thaliana. Expanding the gene count, stress brings about a significant increase of transcripts for which no function is known. Also, we generate insertional mutants that affect stress tolerance in several organisms. More than 400 000 T-DNA tagged lines of A. thaliana have been generated, and lines with altered salt stress responses have been obtained. Integration of these approaches defines stress phenotypes, catalogs of transcripts and a global representation of gene expression induced by salt stress. Determining evolutionary relationships among these genes, mutants and transcription profiles will provide categories and gene clusters, which reveal ubiquitous cellular aspects of salinity tolerance and unique solutions in multicellular species. © 2001 Éditions scientifiques et médicales Elsevier SAS.
Galbraith, D. W., Lambert, G. M., Macas, J., & Dolezel, J. (2001). Analysis of nuclear DNA content and ploidy in higher plants.. Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Chapter 7, Unit 7.6.
PMID: 18770733;Abstract:
This is the first of a series of units discussing the application of cytometry to plant material. Techniques commonly used for mammalian nuclei evaluation need considerable modification to be successful with plant material. David Galbraith and his colleagues bring together many years of knowledge in plant cytometry. Their unit provides detailed protocols on measuring DNA content, ploidy, and cell cycle status of plant tissue using both conventional laser based instruments as well as arc lamp cytometers. This unit provides an excellent starting point for those interested in doing cytometry with plants.
