Jay, E., Harkins, K. R., Galbraith, D. W., & Bohnert, H. J. (1990). Developmentally regulated systemic endopolyploidy in succulents with small genomes. Science, 250(4977), 99-101.
Abstract:
Nuclei from Mesembryanthemum crystallinum (ice plant) exhibit multiple levels of ploidy in every tissue as revealed by flow microfluorometric analysis of isolated nuclei stained with mithramycin. Multiples of the haploid nuclear genome complement (1C) corresponding to 2C, 4C, 8C, 16C, 32C, and 64C were observed. The distribution of nuclei among the different ploidy levels is tissue-specific and in leaves is characteristic of the stage of development. This type of genome organization has been identified in eight other succulent CAM (crassulacean acid metabolism) plant species with small genomes. Multiploidy may be a common property of this type of plant.
Miyazaki, S., Fredricksen, M., Hollis, K. C., Poroyko, V., Shepley, D., Galbraith, D. W., Long, S. P., & Bohnert, H. J. (2004). Transcript expression profiles of Arabidopsis thaliana grown under controlled conditions and open-air elevated concentrations of CO 2 and of O 3. Field Crops Research, 90(1), 47-59.
Abstract:
Microarrays have significantly enhanced our ability to track transcripts and changes in gene expression under different conditions and environments. In order to find determinants of plant performance influenced by atmospheric changes, we tracked transcript profiles in Arabidopsis thaliana (L.) Heynh. grown in a field within SoyFACE. This facility (http://www.soyface.uiuc.edu/ index.htm) consists of a series of open-air rings within which either [CO 2] or [O 3] are elevated above current atmospheric concentrations by 1.5× and 1.2×, respectively. A microarray platform including ∼26,000 DNA elements was used to monitor transcript abundance and changes due to exposure to elevated [CO 2] and [O 3]. Transcripts from plants in growth chambers were further compared with material from plants grown in Free Air Concentration Enrichment (FACE) rings under ambient conditions. Most changes in gene expression were observed between growth chamber and ambient field conditions. Two to four times the number of transcripts were either up- or down-regulated between controlled versus field ambient conditions compared with high versus low [CO 2] or [O 3] contrasts. The differences showed a preponderance of regulated transcripts in categories such as chaperones and general defense reactions, altered (secondary) metabolic functions, redox control, energy provision, protein turnover, signaling and transcription. We discuss the results obtained in a model non-crop plant for their possible relevance in studies with crop species. © 2004 Elsevier B.V. All rights reserved.
Bak, S., Tax, F. E., Feldmann, K. A., Galbraith, D. W., & Feyereisen, R. (2001). CYP83B1, a cytochrome P450 at the metabolic branch paint in auxin and indole glucosinolate biosynthesis in Arabidopsis. PLANT CELL, 13(1), 101-111.
Zhang, X., Zhang, L., Dong, F. C., Gao, J. F., Galbraith, D. W., & Song, C. P. (2001). Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. PLANT PHYSIOLOGY, 126(4), 1438-1448.
Harkins, K. R., & Galbraith, D. W. (1987). Factors governing the flow cytometric analysis and sorting of large biological particles. Cytometry, 8(1), 60-70.
