Barthelson, R. A., Qaisar, U., & Galbraith, D. W. (2010). Functional analysis of the gossypium arboreum genome. Plant Molecular Biology Reporter, 28(2), 334-343.
Abstract:
Gossypium arboreum is an Old World relative of the more commonly cultivated commercial species Gossypium hirsutum, a newer genetic line formed in the New World. G. arboreum has the important property that it can be cultivated in severely hot, dry climates. The genome of G. arboreum has not been completely sequenced, and annotation for the genome is not extensive. We studied the genome of G. arboreum by using cross-species hybridization studies with genomic microarrays for the more annotated species, Arabidopsis thaliana and Oryza sativa. Approximately 30% of the probes on the A. thaliana and O. sativa microarrays were hybridized effectively by target samples prepared from G. arboreum genomic DNA. Many of genes tentatively identified by hybridization function in various levels of the stress response. Cross-species hybridization can provide effective clues as to potentially valuable genes that may be present in a less well-studied species such as G. arboreum. The stress response genes tentatively identified in these studies should provide useful clues for further studies toward the development of hardier strains of cotton. © 2009 Springer-Verlag.
Galbraith, D. W., & Cossarizza, A. (2017). Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, 47, 1584-1797. doi:doi: 10.1002/eji.20164663
Galbraith, D. W., Elumalai, R., & Gong, F. C. (2004). Integrative flow cytometric and microarray approaches for use in transcriptional profiling.. Methods in molecular biology (Clifton, N.J.), 263, 259-280.
PMID: 14976371;Abstract:
Flow cytometry and cell sorting provides unparalleled means for the identification and purification of specific cell types. It is a mature technology having been in existence commercially for the last 25 yr. High-throughput transcriptional profiling methods have emerged relatively recently. These provide the means to characterize efficiently the genome-wide contribution of individual genes to gene expression. A combination of these methods offers the opportunity to explore the relationship between gene expression and the ways in which different cell types are formed and maintained. This chapter provides a review of published methods for analysis of global gene expression within different cell types in complex tissues and organs, and provides practical details concerning microarray fabrication and use based on presynthesized 70-mer oligonucleotide array elements.
Galbraith, D. W., & Birnbaum, K. (2006). Global studies of cell type-specific gene expression in plants. Annual Review of Plant Biology, 57, 451-475.
PMID: 16669770;Abstract:
Technological advances in expression profiling and in the ability to collect minute quantities of tissues have come together to allow a growing number of global transcriptional studies at the cell level in plants. Microarray technology, with a choice of cDNA or oligo-based slides, is now well established, with commercial full-genome platforms for rice and Arabidopsis and extensive expressed sequence tag (EST)-based designs for many other species. Microdissection and cell sorting are two established methodologies that have been used in conjunction with microarrays to provide an early glimpse of the transcriptional landscape at the level of individual cell types. The results indicate that much of the transcriptome is compartmentalized. A minor but consistent percentage of transcripts appear to be unique to specific cell types. Functional analyses of cell-specific patterns of gene expression are providing important clues to cell-specific functions. The spatial dissection of the transcriptome has also yielded insights into the localized mediators of hormone inputs and promises to provide detail on cell-specific effects of microRNAs. Copyright © 2006 by Annual Reviews. All rights reserved.
Ozkan, H., Tuna, M., & Galbraith, D. W. (2006). No DNA loss in autotetraploids of Arabidopsis thaliana. Plant Breeding, 125(3), 288-291.
Abstract:
To address the issue of genome evolution in autopolyploids and particularly to investigate whether rapid sequence elimination also occurs in autopolyploids as in allopolyploids, amplified fragment length polymorphism (AFLP) fingerprinting was employed to examine a large number of genomic loci in F 1 hybrids between two different autotetraploids of Arabidopsis thaliana accessions, namely Ler and Col. Using this approach, perfect additivity in the F1 hybrids was found between the newly-formed autopolyploids when compared with their parental lines. Using flow cytometry, the study was extended in a quantitative manner, in which the nuclear DNA contents in one autotetraploid A. thaliana accession Ler, was determined. The increase in genome size of the autotetraploid line was additive. Taken together, no evidence was found for genome size reduction due to autopolyploidization of A. thaliana. The results indicating that there was no DNA loss in autotetraploid A. thaliana suggest that a different type of genome evolution may occur in autopolyploids during the initial stages of their formation when compared with allopolyploids. © 2006 Blackwell Verlag.
