Doore, S. M., Baird, C., The 2012 University of Arizona Virology Undergraduate Lab, ., Roznowski, A. P., & Fane, B. A. (2014). The evolution of genes within genes and the control of DNA replication in Microviruses. Molecular Biology and Evolution, 31(6), 1421-1431.
Approximately 20% of the data in this manuscript was generated by students enrolled in the 2012 Summer Virology Lab Course.
Fane, B. A., & Hayashi, M. (1991). Second-site suppressors of a cold-sensitive prohead accessory protein of bacteriophage ΦX174. Genetics, 128(4), 663-671.
PMID: 1833267;PMCID: PMC1204541;Abstract:
This study describes the isolation of second-site suppressors which correct for the defects associated with cold-sensitive (cs) prohead accessory proteins of bacteriophage ΦX174. Five phenotypically different suppressors were isolated. Three of these suppressors confer novel temperature-sensitive (ts) phenotypes. They were unable to complement a ts mutation in gene F which encodes the major coat protein of the phage. All five suppressor mutations confer nucleotide changes in the gene F DNA sequence. These changes define four amino acid sites in the gene F protein. Three suppressor mutations placed into an otherwise wild-type background display a cold resistant phenotype in liquid culture infections when compared to a wild-type ΦX174 control.
Fane, B. A., & Prevelige Jr., P. E. (2003). Mechanism of scaffolding-assisted viral assembly. Advances in Protein Chemistry, 64, 259-299.
Fane, B. A., Head, S., & Hayashi, M. (1992). Functional relationship between the J proteins of bacteriophages φX174 and G4 during phage morphogenesis. Journal of Bacteriology, 174(8), 2717-2719.
PMID: 1532571;PMCID: PMC205913;Abstract:
The functions of the small DNA-binding protein, gpJ, of bacteriophages φX174 and G4 were examined by in vivo cross-complementation and sucrose gradient sedimentation. The morphogenetic roles of the two proteins may differ. The φX174 J protein may be required for the formation or stabilization of the φX174 prohead.