Ubando, A.T., Cuello, J.L., Culaba, A.B., El-Halwagi, M.M., Tan, R.R. 2014. Multi-Regional Multi-Objective Optimization of an Algal Biofuel Polygeneration Supply Chain with Fuzzy Mathematical Programming. American Society of Mechanical Engineers (ASME) Digital Collection: Paper No. IMECE2013-66236, V012T13A045; 10 pages, doi:10.1115/IMECE2013-66236, ISBN: 978-0-7918-5641-3. Refereed.
Ubando, A.T., Culaba, A.B., Aviso, K.B., Tan, R.R., Cuello, J.L., Ng, D.K.S., El-Halwagi, M.M., (2015). Fuzzy Mathematical Programming Approach in the Optimal Design of an Algal Bioenergy Park. Chemical Engineering Transactions. Vol. 45. ISBN 978-8895608, ISSN 2283-9216.
A new strategy in photosynthetic hydrogen (photo-H(2)) production from green algae was developed based on theory and successfully demonstrated. The new strategy applied a spectral-selective photosystem I (PSI) activating/photosystem II (PSII) deactivating radiation (or PSI light) that would drive a steady flow of electrons in the electron transport chain for delivery to hydrogenase for photo-H(2) production, but would reduce oxygen production through water photolysis below the respiratory oxygen consumption so that an anoxic condition would be maintained as required by hydrogenase. Implementing the strategy by using a PSI light (692 nm peak, 680-700 nm) on Chlamydomonas reinhardtii cells resulted in relatively sustained photo-H(2) production (total of 0.108 mL H(2)mg(-1)Chl, exceeding 0.066 mL H(2)mg(-1)Chl under white light). The strategy also proved successful and convenient in allowing cells to alternately switch between photo-H(2) production and a recovery period by simply turning on or off the PSI light.