Judith K Brown

Judith K Brown

Professor, Plant Science
Regents Professor, Plant Sciences
Research Associate Professor, Entomology
Professor, Entomology / Insect Science - GIDP
Professor, BIO5 Institute
Member of the General Faculty
Member of the Graduate Faculty
Primary Department
Department Affiliations
Contact
(520) 621-1402

Work Summary

Unravel the phylodynamics and transmission-specific determinants of emerging plant virus/fastidious bacteria-insect vector complexes, and translate new knowledge to abate pathogen spread in food systems.

Research Interest

Judith Brown, PhD, and her research interests include the molecular epidemiology of whitefly-transmitted geminiviruses (Begomoviruses, Family: Geminiviridae), the basis for virus-vector specificity and the transmission pathway, and the biotic and genetic variation between populations of the whitefly vector, B. tabaci, that influence the molecular epidemiology and evolution of begomoviruses. Keywords: Plant viral genomics, emergent virus phylodynamics, functional genomics of insect-pathogen interactions

Publications

Bouharroud, R., Hanafi, A., Brown, J. K., & Serghini, M. A. (2006). Resistance and cross-resistance of Bemisia tabaci to three commonly used insecticides in the tomato greenhouses of the Souss Valley of Morocco. European Journal of Scientific Research, 14(4), 587-594.

Abstract:

In this study, 10 populations of whiteflies B. tabaci were collected from tomato greenhouses in the Souss valley of Morocco and bioassayed for resistance to three commonly used insecticides (Imidacloprid, Thaimethoxam and Methomyl). Using Leaf-Dip bioassay, we concluded that all populations tested were resistant to Imidacloprid and Thiamethoxam. The resistance factors registered were varied between 2 to 39 and 2 to 12, respectively. The levels of resistance to Methomyl were slightly low and showed a moderate variation between 1 and 4. The analyses of correlation showed a very significant cross-resistance of B. tabaci to this two Neonicotinoids. The predominant biotype noted in this study is biotype Q with the presence of some B. tabaci populations expressed a variant of biotype Q. © EuroJournals Publishing, Inc. 2006.

Fauquet, C. M., Briddon, R. W., Brown, J. K., Moriones, E., Stanley, J., Zerbini, M., & Zhou, X. (2008). Geminivirus strain demarcation and nomenclature. Archives of Virology, 153(4), 783-821.

PMID: 18256781;Abstract:

Geminivirus taxonomy and nomenclature is growing in complexity with the number of genomic sequences deposited in sequence databases. Taxonomic and nomenclatural updates are published at regular intervals (Fauquet et al. in Arch Virol 145:1743-1761, 2000, Arch Virol 148:405-421, 2003). A system to standardize virus names, and corresponding guidelines, has been proposed (Fauquet et al. in Arch Virol 145:1743-1761, 2000). This system is now followed by a large number of geminivirologists in the world, making geminivirus nomenclature more transparent and useful. In 2003, due to difficulties inherent in species identification, the ICTV Geminiviridae Study Group proposed new species demarcation criteria, the most important of which being an 89% nucleotide (nt) identity threshold between full-length DNA-A component nucleotide sequences for begomovirus species. This threshold has been utilised since with general satisfaction. More recently, an article has been published to clarify the terminology used to describe virus entities below the species level [5]. The present publication is proposing demarcation criteria and guidelines to classify and name geminiviruses below the species level. Using the Clustal V algorithm (DNAStar MegAlign software), the distribution of pairwise sequence comparisons, for pairs of sequences below the species taxonomic level, identified two peaks: one at 85-94% nt identity that is proposed to correspond to "strain" comparisons and one at 92-100% identity that corresponds to "variant" comparisons. Guidelines for descriptors for each of these levels are proposed to standardize nomenclature under the species level. In this publication we review the status of geminivirus species and strain demarcation as well as providing updated isolate descriptors for a total of 672 begomovirus isolates. As a consequence, we have revised the status of some virus isolates to classify them as "strains", whereas several others previously classified as "strains" have been upgraded to "species". In all other respects, the classification system has remained robust, and we therefore propose to continue using it. An updated list of all geminivirus isolates and a phylogenetic tree with one representative isolate per species are provided. © 2008 Springer-Verlag.

Leke, W. N., Njualem, D. K., Nchinda, V. P., Ngoko, Z., Zok, S., Ngeve, J. M., Brown, J. K., & Kvarnheden, A. (2009). Molecular identification of Maize streak virus reveals the first evidence for a subtype A1 isolate infecting maize in Cameroon. Plant Pathology, 58(4), 782-.
Kirk, A. A., Lacey, L. A., Roditakis, N., & Brown, J. K. (1993). The status of Bemisia tabaci (Hom.: Aleyrodidae), Trialeurodes vaporariorum (Hom.: Aleyrodidae) and their natural enemies in Crete. Entomophaga, 38(3), 405-410.

Abstract:

The non «B» biotype of Bemisia tabaci (Gennadius) is recorded for the first time in Crete in 1992, in the north east and south east of the island. Trialeurodes vaporariorum (Westwood) is the predominant whitefly on plants in the north and west of the island. Three surveys of Crete were made in 1992 and 1993 for natural enemies of B. tabaci and T. vaporariorum and resulted in the collection of 4 species of Encarsia, (plus a number of species that are unidentifiable at this time), an Eretmocerus sp. (unidentifiable at this time) and a fungal pathogen, Paecilomyces farinosus (Dickson Ex Fries) Brown & Smith. Encarsia adrianae was identified from T. vaporariorum; which constitutes its most westerly distribution point and a new host record respectively. B. tabaci and T. vaporariorum were found on horticultural crops, ornamentals and weeds. Populations of both whitefly species were severely depleted on field hosts throughout the island during the winter of 1992/93. Climatic constraints, competition with T. vaporariorum in otherwise suitable niches, effective natural enemies and an observed low level of polyphagy may explain the present limited distribution of the non «B» biotype of B. tabaci in Crete. © 1993 Lavoisier Abonnements.

Brown, J. K. (2016). First report of papaya naturally-infected with the introduced Tomato yellow leaf curl virus-IL. (Short Report). Plant Dis., 100, 1959. doi:http://dx.doi.org/10.1094/PDIS-04-16-0469-PDN.

Alabi, O.J., Al Rwahnih, M., Brown, J.K., Idris, A.M., Gregg, L., Kmieciak, E., Sétamou, M., and Jifon, J.L. 2016. First report of papaya naturally-infected with the introduced Tomato yellow leaf curl virus-IL. Plant Dis. 100:1959. http://dx.doi.org/10.1094/PDIS-04-16-0469-PDN.