Holtzer, H., Forry-Schaudies, S., Antin, P., Dubyak, G., & Nachmias, V. (1985). Induction of incoordinate synthesis of muscle proteins by the tumor promoter TPA and the carcinogen EMS.. Advances in Experimental Medicine and Biology, 182, 179-192.
Darnell, D. K., Kaur, S., Stanislaw, S., Konieczka, J. H., Yatskievych, T. A., & Antin, P. B. (2007). Erratum: MicroRNA expression during chick embryo development (Developmental Dynamics 235 (3156-3165)). Developmental Dynamics, 236(1), 333-.
Kazmierski, S. T., Antin, P. B., Witt, C. C., Huebner, N., McElhinny, A. S., Labeit, S., & Gregorio, C. C. (2003). The complete mouse nebulin gene sequence and the identification of cardiac nebulin. Journal of Molecular Biology, 328(4), 835-846.
BIO5 Collaborators
Parker B Antin, Carol C Gregorio
PMID: 12729758;Abstract:
Nebulin is a giant (Mr 750-850kDa), modular sarcomeric protein proposed to regulate the assembly, and to specify the precise lengths of actin (thin) filaments in vertebrate skeletal muscles. Nebulin's potential role as a molecular template is based on its structural and biochemical properties. Its central ∼700kDa portion associates with actin along the entire length of the thin filament, its N-terminal region extends to thin filament pointed ends, and ∼80kDa of its C-terminal region integrates within the Z-line lattice. Here, we determined the exon/intron organization of the entire mouse nebulin gene, which contains 165 exons in a 202kb segment. We identified 16 novel exons, 15 of which encode nebulin-repeat motifs (12 from its central region and 3 from its Z-line region). One novel exon shares high sequence homology to the 20 residue repeats of the tight-junction protein, ZO-1. RT-PCR analyses revealed that all 16 novel exons are expressed in mouse skeletal muscle. Surprisingly, we also amplified mRNA transcripts from mouse and human heart cDNA using primers designed along the entire length of nebulin. The expression of cardiac-specific nebulin transcripts was confirmed by in situ hybridization in fetal rat cardiomyocytes and in embryonic Xenopus laevis (frog) heart. On the protein level, antibodies specific for skeletal muscle nebulin's N and C-terminal regions stained isolated rat cardiac myofibrils at the pointed and barbed ends of thin filaments, respectively. These data indicate a conserved molecular layout of the nebulin filament systems in both cardiac and skeletal myofibrils. We propose that thin filament length regulation in cardiac and skeletal muscles may share conserved nebulin-based mechanisms, and that nebulin isoform diversity may contribute to thin filament length differences in cardiac and skeletal muscle. © 2003 Elsevier Science Ltd. All rights reserved.
Schoenwolf, G., Antin, P., Padhye, S., & Pendleton, A. (2008). DD is fully compliant (and then some) with NIH and other funding agencies. Developmental Dynamics, 237(9), 2283-.
Antin, P. B., Pier, M., Sesepasara, T., Yatskievych, T. A., & Darnell, D. K. (2010). Embryonic expression of the chicken Krüppel-like (KLF) transcription factor gene family. Developmental dynamics : an official publication of the American Association of Anatomists, 239(6), 1879-87.
The Krüppel-like transcription factors (KLF) are zinc finger proteins that activate and suppress target gene transcription. Although KLF factors have been implicated in regulating many developmental processes, a comprehensive gene expression analysis has not been reported. Here we present the chicken KLF gene family and expression during the first five days of embryonic development. Fourteen chicken KLF genes or expressed sequences have been previously identified. Through synteny analysis and cDNA mapping, we have identified the KLF9 gene and determined that the gene presently named KLF1 is the true ortholog of KLF17 in other species. In situ hybridization expression analyses show that in general KLFs are broadly expressed in multiple cell and tissue types. Expression of KLFs 3, 7, 8, and 9, is widespread at all stages examined. KLFs 2, 4, 5, 6, 10, 11, 15, and 17 show more restricted patterns that suggest multiple functions during early stages of embryonic development.
